CK-PAN - Pan Cytokeratin

Control type	Positive tissue control High expression level	Positive tissue control Low expression levels	Negative tissue control
Tissue	Tonsil	Liver	Tonsil
Description	Virtually all squamous epithelial cells must show a moderate to strong, distinct cytoplasmic staining reaction (Demonstration of CK-HMW).	Virtually all hepatocytes must show an at least weak to moderate cytoplasmic staining reaction typically with a membrane accentuation (Demonstration of CK-LMW). Note epithelial cells of bile ducts will show a strong cytoplasmic staining reaction and cannot be used to evaluate analytical sensitivity (Demonstration of CK-LMW).	No staining reaction in lymphocytes. Note interstitial/interdigitating reticulum cells may show a weak to moderate cytoplasmic staining reaction due to expression of CK 8 and 18.
Example	Virtually all squamous epithelial cells must show a moderate to strong, distinct cytoplasmic staining reaction (Demonstration of CK-HMW).	Virtually all hepatocytes must show an at least weak to moderate cytoplasmic staining reaction typically with a membrane accentuation (Demonstration of CK-LMW). Note epithelial cells of bile ducts will show a strong cytoplasmic staining reaction and cannot be used to evaluate analytical sensitivity (Demonstration of CK-LMW).	No staining reaction in lymphocytes. Note interstitial/interdigitating reticulum cells may show a weak to moderate cytoplasmic staining reaction due to expression of CK 8 and 18.

Control type

Positive tissue control
High expression level

Positive tissue control
Low expression levels

Negative tissue control

Tissue

Tonsil

Liver

Tonsil

Description

Virtually all squamous epithelial cells must show a moderate to strong, distinct cytoplasmic staining reaction (Demonstration of CK-HMW).

Virtually all hepatocytes must show an at least weak to moderate cytoplasmic staining reaction typically with a membrane accentuation (Demonstration of CK-LMW).

Note epithelial cells of bile ducts will show a strong cytoplasmic staining reaction and cannot be used to evaluate analytical sensitivity (Demonstration of CK-LMW).

No staining reaction in lymphocytes.

Note interstitial/interdigitating reticulum cells may show a weak to moderate cytoplasmic staining reaction due to expression of CK 8 and 18.

Example

Virtually all squamous epithelial cells must show a moderate to strong, distinct cytoplasmic staining reaction (Demonstration of CK-HMW).

Virtually all hepatocytes must show an at least weak to moderate cytoplasmic staining reaction typically with a membrane accentuation (Demonstration of CK-LMW).

Note epithelial cells of bile ducts will show a strong cytoplasmic staining reaction and cannot be used to evaluate analytical sensitivity (Demonstration of CK-LMW).

No staining reaction in lymphocytes.

Note interstitial/interdigitating reticulum cells may show a weak to moderate cytoplasmic staining reaction due to expression of CK 8 and 18.