Control type | Positive tissue control High expression level | Positive tissue control Low expression levels | Negative tissue control |
---|---|---|---|
Tissue | Appendix/colon | Liver | Appendix/colon |
Description | Virtually all lymphocytes (incl intra-epithelial T-cells), endothelial cells, stromal cells and macrophages must show a moderate to strong, distinct predominantly cytoplasmic staining reaction. | Virtually all Kupffer cells and endothelial cells lining sinusoids must show an at least weak to moderate predominantly cytoplasmic staining reaction. | No staining reaction in epithelial cells in the epithelial mucosa. |
Example | Virtually all lymphocytes (incl intra-epithelial T-cells), endothelial cells, stromal cells and macrophages must show a moderate to strong, distinct predominantly cytoplasmic staining reaction. | Virtually all Kupffer cells and endothelial cells lining sinusoids must show an at least weak to moderate predominantly cytoplasmic staining reaction. | No staining reaction in epithelial cells in the epithelial mucosa. |